In vitro & In vivo models
TrGET preclinical assay platform
TrGET is a preclinical platform, performing and developing in vitro (since 2005) and in vivo (since 2008) testing of target genes involved in tumorogenesis or anti-tumoral therapeutics.
Great efforts are provided towards technological developments, including assay, tool and both cell and animal proposed models.
TrGET offers a variety of flexible and customizable services, with privileged development of bioluminescence-based technologies: in vitro assays for proliferation/cytotoxicity, survival/apoptosis, clonogenicity, migration and angiogenesis; in vivo (non invasive intravital imaging) of tumor volume, time to progression or doubling, tumoral dissemination (blood, bone marrow, spleen,…) and metastasis, survival; and tissue collection, preparation and preservation.
The data are statistically analyzed and additional investigations can be provided.
Management & contact: Dr Yves Collette
Date of creation: 2005
Staff (CRCM and others): 1 scientist, 1 enginner and 2 technicians
The cell culture platform
The Cell Culture Platform comprises four laboratories allowing the use and manipulation of organisms belonging to class 2 biohazard, and a satellite laboratory that houses equipment used in cell biology (electroporation units, cell counters, plate readers, fluorescence digital microscope, etc.).
It is divided into affected areas based on technical requirements and biological risk, which can be accessed by a procedure of online booking.
The platform provides, for the CRCM and the research units in the TPR2 building, facilities and services for eukaryotic cell culture according to the highest Quality Standards. It may potentiate collaborations with university research structures or private organizations, both nationally and internationally.
- Providing cell culture facilities, fully controlled and consistent with Good Cell Culture Practices
- Training in cell culture
- Participation in research projects using cell culture
- Technical expertise
- Technological survey
The organization of the Platform is coordinated by an engineer from Inserm-U1068. The Quality Management System complies with ISO9001. The objective is to achieve continuous improvement of service to meet users’ expectations in accordance with laws and regulations.
An internal regulation lays down rules for use of the Platform (experimenter skills, booking of devices, maintenance of equipment, waste management, etc.).
The operating budget is subject to a use agreement, and ensures all operating expenses and specific equipment.
Location: Building TPR2, Campus of Marseille-Luminy
The animal facility provides the basic animal breeding and maintenance service for genetically modified and wild type mouse strains. The animals are raised in the Specific and Opportunistic Pathogen Free (SOPF) areas. Most of the mouse strains are housed in individually ventilated caging systems and are handled under sterile conditions. The own full time staff is providing expert technical assistance on mouse strain genotyping and injecting ES cells.
The animal facility of the IPC campus comprises 250 sq. m. with 5 rooms, and houses a mouse genetics lab, bioluminescence and fluorescence intra-vital imaging System (Photon Imager, BioSpace Lab) and state of the art equipment for measurements of haematological parameters.
External services to access at the in vivo imaging system are available via the TrGET facility.
The CRCM animal facility is a member of an animal facility network shared between two « Instituts Federatifs de Recherche »: Cancer and Immunology Institute of Marseille and Developmental Biology Institute of Marseille-Luminy.
Date of creation: 1993
Staff (CRCM and others): 2 Scientists, 4 technicians
The animal facility PSEA (Animal Experimentation and Stabulation Plateform) of the Luminy campus is spreading out on 250 m2. The surface is divided in 4 A1+ Housing/experimentation areas (with one specifically dedicated to rederivation through aseptic hysterectomy) and one A2 housing/experimentation area. These areas can actually contain 700 and 244 cages respectively, with the ability to enhance A1+ area from 700 to 1200 cages. This animal facility also contains one storage area dedicated to A1+ autoclaved materials and one storage area dedicated to A2 autocalved materials, one circulating area as well as one 30m2 experimental area with technical tools necessary for the animal facility users (among them, a bioluminescent and fluorescent intravital imaging system, the PhotonImage from Biospace Lab). This animal facility is coordinated following strict internal rules by which the main housing and experimental procedures are defined. The PSEA is directed by a technical/personal manager and scientific/technical board. Moreover, in agreement with ongoing rules, a pharmacological manager and a “well-being” comity are also implicated in general managing.
The PSEA animal facility is also a member of the animal facility network shared between two « InstitutsFederatifs de Recherche »: Cancer and Immunology Institute of Marseille and Developmental Biology Institute of Marseille-Luminy.
Management & contact: Richard Tomasini
Date of creation: 2012
Staff (CRCM and others): 1 Scientist, 2 technicians
The transgenesis laboratory
The transgenesis laboratory is dedicated to the engineering of genetically modified mice by insertional mutagenesis or by homologous recombination (HR) for the research teams of the CRCM. It consists of a microinjection bench is equipped with an inverted microscope coupled to two micromanipulation devices and two micro-vacuum-pumps, a laminar flow hood and a CO2 incubator allowing the mycoplasm-free culture of Embryonic Stem (ES) cells. A phenotypic bench equipped with two binocular lenses is also available for the analysis of mutant mice.
The transgenesis lab staff handles the culture of ES cells and neo-resistant-Mouse Embrynoic Fibroblasts (MEFs) and the preparation of the relevant culture media. The staff is available to train and assist CRCM scientists and students for ES cell and HR technology (vector construction, electroporation and selection of ES cells for HR). It also has contacts with several European and international ES cell libraries.
The transgenesis lab staff produces mutant mice from the ES cells supplied by CRCM scientists (blastocyst extraction, microinjection of ES cells and re-implantation in carrier mice). The staff also breads and produces all animals required for the injections (male sterilization, mating…) and it generates the mice lineages resulting from ES cell injections.
Staff: 1 engineer, 1 technician