24 months post-doctoral offer in mouse genetic and molecular and cellular biology

 

 

 

24 months post-doctoral offer in mouse genetic

and molecular and cellular biology

Epithelial stem cell and cancer laboratory

Centre de Recherche en Cancérologie de Marseille (CRCM),

27 Bd Lei Roure, 13009 Marseille, FRANCE

Dr. Géraldine Guasch, geraldine.guasch-grangeon(at)inserm(dot)fr

https://guaschresearch.info/

 

Title of the project: Investigating mechanisms of anorectal pre-cancerous development in a model of intra-epithelial neoplasia

 

Context: In humans and mouse, epithelial tumours frequently develop in transition zones which represent the junction between two types of epithelia such as between the anus and the rectum (McCauley et al., 2017). We have recently developed a mouse model of human anorectal tumors at transition zones to follow the early steps of tumor progression from healthy to pre-neoplastic stage. In these mice, the transformation occurs only at a restricted area allowing to clearly address the cellular and molecular mechanisms in both the epithelial and the immune compartments that contribute to the establishment of the tumor.

 

Project: The goal of the proposal is to characterize the pre-tumoral immune environment of future transition zone tumors and understand how its presumed specific characteristics modulate the susceptibility to tumor development.

 

The project will be divided in two axes:

1.                 Identify at the single cell level the malignant events that could represent early neoplastic changes occurring during the hyperplasic to pre-malignant transition at the anorectal TZ

2.                 Investigate the role of T cell anal transition zone stromal fractions in mediating epithelial hyperplasia to pre-neoplasic progression

This project is based on the use of a mouse model and a three-dimensional organoid culture of transition zone to examine in more depth at a single-cell level whether specialized subsets of lymphocytes present at transition zones are directly linked to susceptibility to carcinogenesis.

 

 

Required expertise: Interested applicants should have a PhD degree in Biology, Genetics, Biochemistry, or an equivalent field as well as a background in molecular cell biology techniques. Ideally, these will include experience with mouse genetics, mammalian cell culture, cell imaging and cell sorting (Flow cytometry). Experience in immunology and bioinformatics is welcome, but not essential. Prospective candidates will be part of a highly collaborative research team and expected to be highly motivated.

 

Contact:

Send your candidature including a CV, a motivation letter and contact information for academic references letters to  geraldine.guasch-grangeon(at)inserm(dot)fr

 

Dr. Géraldine Guasch, Ph.D, HDR

Leader of the Epithelial Transition Zone team

Centre de Recherche en Cancérologie de Marseille (CRCM),

27 Bd Lei Roure, 13009 Marseille, France

 

Main publications:

 

Matrka MC., Cimperman KA., Haas SR., Guasch G., Ehrman LA., Waclaw RR., Wikenheiser-Brokamp KA, Wells SI. Dek Overexpression in Murine Epithelia Increases Overt Esophageal Squamous Cell Carcinoma Incidence. Plos Genetics. 2018 14(3):e1007227.

 

McCauley, HA., Chevrier V., Birnbaum D., Guasch G.  De-repression of ELMO1 in cancer stem cells drives progression of TGFβ-deficient squamous cell carcinoma from transition zones. ELife. 2017, pii: e22914.

 

McCauley, H.A. and Guasch G. Three Cheers For The Goblet Cell: Maintaining Homeostasis In Mucosal Epithelia. Trends In Molecular Medicine. 2015, 21:492-503.

 

McCauley H.A, Liu C-Y, Attia A, Wikenheiser-Brokamp KA, Zhang Y, Whitsett JA, and Guasch G. TGFβ signaling inhibits goblet cell differentiation via SPDEF in the conjunctival epithelium. Development. 2014 141:4628-4639.               

CDD Chimiste en synthèse chimique organique/médicinale

30 months post doc fellowship of metabolic targeting of pancreatic cancer

see attached ad

The CRCM is recruiting new group leaders

POST-DOC position in EV molecular/cellular biology

 

The Cancer Research Center Marseille (CRCM) common goal is to advance our understanding of the biology of cancer and to improve treatment. CRCM combines access to high education in immunology and oncology, state-of-the-art technological support (platforms & resources) and a collegial set of teams offering a fertile and outstanding environment to develop fundamental, translational or clinical project. Our multidisciplinary teams of about 400 researchers are housed on the campus of the Institut Paoli-Calmettes, one of the largest university-affiliated cancer centers in France with 1,500 staff-members (http://www.institutpaolicalmettes.fr/en/research/).

 

Description

At CRCM, the team of Pascale Zimmermann is investigating novel pathways controlling cell signaling and cell-cell communication and exploring their potential benefit for the cancer field. The team provided major contributions to our understanding of the importance of membrane trafficking for the biology of heparan sulfate proteoglycans, the role of phospholipids in the function of PDZ proteins and more recently the molecular mechanisms of exosome biogenesis (e.g. Baietti et al., Nat Cell Biol 2012; Ghossoub et al., Nat Commun 2014; Imjeti et al., PNAS 2017).

Job description

We are recruiting a young post-doc interested in understanding the mechanisms controlling the molecular and functional diversity of extracellular vesicles and in particular at investigating the crosstalk between membrane scaffolds tetraspanins, syndecans and PDZ proteins. Financial support is foreseen for up to 3 years and candidate are encouraged to apply for competitive fellowships (EMBO, Marie-Curie or French agencies). This work is supported by the ANR project SynTEV which is a joined effort with the group of Clotilde Théry and Eric Rubinstein.

Profile

    PhD in Biochemistry, Molecular Biology, Cell Biology or equivalent

    Strong background in imaging methods (microscopy and/or FACS) and protein/membrane work   

    Proficient in written and spoken English

    Strong organizational and interpersonal skills

How to apply?

Motivated candidates are asked to apply by email to pascale.zimmermann(at)inserm(dot)fr (subject Post-Doc SynTEV) and to send applications including CV, research experience, and at least two names and contact information for references.

 

For more information, you can contact Pascale Zimmermann (by email).

Ingénieur d’étude dans le groupe ‘’Cellules Souches Epitheliales et Cancer’’

Poste CDD de 18 mois à partir de Janvier 2019 : Ingénieur d’étude dans le groupe ‘’Cellules Souches Epitheliales et Cancer’’ au Centre de Recherche en Cancérologie de Marseille (http://crcm.marseille.inserm.fr/equipesde-recherche/emmanuelle-charafe-jauffretet-christophe-ginestier/). Le/La candidat(e) sera impliqué(e) dans la culture et l’imagerie d’organoïdes de divers organes murins et humains. Une formation M2 Pro ou M2 avec une expertise en culture cellulaire et imagerie est recherchée (le/la candidat(e) ne doit pas avoir été formé(e) à l’université d'Aix Marseille AMU)

Contacter Géraldine Guasch

Propositions de sujets de stage de Master 2 (2019-2020)

Formulaire d'inscription au Master 2 Meeting Day

Miscellaneous jobs

No open position

The CRCM is always searching for dedicated and motivated staff.

Do not hesitate to contact us.